सीएसबी - केंद्रीय रेशम उत्पादन अनुसंधान एवं प्रशिक्षण संस्थान , मैसूरु

मनंदावाड़ी रोड, श्रीरामपुरा, केंद्रीय रेशम बोर्ड - वस्त्र मंत्रालय - भारत सरकार

CSB – Central Sericultural Research & Training Institute (CSRTI), Mysuru

Manandavadi Road, Srirampura, Central Silk Board, Ministry of Textiles, Government of India

Swachh Bharat Logo
Designation :

Scientist-B


Present Posting :

CSB - Central Sericultural Research & Training Institute (CSRTI), Mysuru


Specialization :

Molecular Biology & Biotechnology


Qualification :
University / Degree Details Year
University of Agricultural Sciences, GKVK, Bangalore
M.Sc., (Agri) Plant Biotechnology
Thesis title: CRISPR/Cas9 mediated genome editing in Bactrocera dorsalis (Hendel) (Tephritidae: Diptera) and Maruca vitrata (Geyer) (Crambidae: Lepidoptera)
2021–2023
University of Agricultural Sciences, GKVK, Bangalore
B.Tech., Biotechnology
2017–2021
Research Work Experience :
University of Agricultural Sciences, GKVK , Bangalore, India

Master’s Thesis | Advisors: Dr. Nagesha S N & Dr. R. Asokan

CRISPR/Cas9 mediated genome editing in Bactrocera dorsalis (Hendel) and Maruca vitrata (Geyer) in collaboration with ICAR-IIHR.

Work Focused On:
  1. Cloning and characterization of target genes such as transformer2 and vitellogenin.
  2. Designing off-target minimized sgRNAs, in vitro transcription and confirmation using cleavage assay.
  3. Delivery of RNP (Ribonucleoprotein) and mutation analysis through phenotyping and genotyping.
ICAR - Indian Institute of Horticultural Research , Bangalore, India

Advisor: Dr. R. Asokan (Principal Scientist)

Worked on vitellogenin gene in Bactrocera dorsalis. Characterized the gene and designed sgRNA for mutagenesis.

Hands-on Training in Basic Bioinformatics , COA, Hassan, India

Conducted in silico analysis of SARS-CoV2 spike protein.

Hands-on Training in Synthetic Biology , IBAB, Bangalore, India

Details of research Projects :

N/A


Research publications :
Publications Numbers
Full length Research Papers 14
Review Papers -
Research Shortnotes -
Books -
Book Chapters -
Research Abstracts 1
Popular Articles 7
TV / Radio Programmes -
NCBI Gene Accessions 236

Best publications :
  1. Pradeep, C., Ashok, K., VinayKumar, T.M., Babu, S.S., Subhaharan, K., Nagesha, S.N. and Asokan, R., 2025. Integrative functional validation of pheromone binding proteins in Spodoptera frugiperda via in silico and RNAi approaches. International Journal of Biological Macromolecules, p.148440. (IF: 8.5, NAAS: 14.50)
  2. Parvathy, M., Pradeep, C., Nagesha, S., Asokan, R. And Harinikumar, K., 2024. Selection and in vitro Validation of Highly Efficient Single Guide RNAs Fly, Zeugodacus cucurbitae (Coquillett). Mysore Journal of Agricultural Sciences, 58(3). (NAAS: 4.63)
  3. Sujatha, P.M., Karuppannasamy, A., Chalapathi, P., Dhawane, Y., Narasimhappa, N.S., Narayanappa, A.C., Munikrishnappa, V.K.T., Chikmagalur Nagaraja, B., Thalooru, S., Kesavan, S. and Maligeppagol, M., 2024. CRISPR/Cas9 Ribo nucleoprotein complex-mediated editing of the OBP13 gene affected the response of male Bactrocera dorsalis (Diptera: Tephritidae) to methyl eugenol. Journal of Applied Entomology. (IF: 2, NAAS: 7.70)
  4. Nagesha, S.N., Ramesh, B.N., Pradeep, C., Shashidhara, K.S., Ramakrishnappa, T., Krishnaprasad, B.T., Jnanashree, S.M., Manohar, M., Arunkumar, N., Patel, D. and Rakesh, T.V., 2022. SARS-CoV 2 spike protein S1 subunit as an ideal target for stable vaccines: A bioinformatic study. Materials Today: Proceedings, 49, pp.904-912. (IF: 2.84)
  5. Ashok, K., Bhargava, C.N., Pradeep, C., Pradhan, S.K., Jha, G.K., Maligeppagol, M., Shivanna, B. and Asokan, R., 2024. Toward the genetic suppression of Bactrocera dorsalis (Diptera: Tephritidae) through CRISPR/Cas9-mediated editing of spermatogenesis-related genes, Tssk1 and topi for imparting male sterility. Annals of the Entomological Society of America, 117(5), pp.270-279. (IF: 2.12, NAAS: 9)
  6. Ashok, K., Bhargava, C.N., Asokan, R., Pradeep, C., Kennedy, J.S., Manamohan, M. and Rai, A., 2023. CRISPR/Cas9 mediated mutagenesis of the major sex pheromone gene, acyl-CoA delta-9 desaturase (DES9) in fall armyworm Spodoptera frugiperda (JE Smith)(Lepidoptera: Noctuidae). International Journal of Biological Macromolecules, 253, p.126557. (IF: 8.5, NAAS: 13.70)
  7. Ashok, K., Bhargava, C.N., Asokan, R., Pradeep, C., Pradhan, S.K., Kennedy, J.S., Balasubramani, V., Murugan, M., Jayakanthan, M., Geethalakshmi, V. and Manamohan, M., 2023. CRISPR/Cas9 mediated editing of pheromone biosynthesis activating neuropeptide (PBAN) gene disrupts mating in the Fall armyworm, Spodoptera frugiperda (JE Smith)(Lepidoptera: Noctuidae). 3 Biotech, 13(11), p.370. (IF: 3.26, NAAS: 8.90)
  8. Ashok, K., Bhargava, C.N., Asokan, R., Pradeep, C., Kennedy, J.S., Rai, A. and Manamohan, M., 2023. First report on the utility of pupal case for early determination of CRISPR/Cas9 ribonucleoprotein mediated genomic edits in the oriental fruit fly, Bactrocera dorsalis (Hendel)(Tephritidae: Diptera). Archives of Insect Biochemistry and Physiology, 113(4), p.e22024. (IF: 2.05, NAAS: 7.50)
  9. Chikmagalur Nagaraja, B., Karuppannasamy, A., Ramasamy, A., Cholenahalli Narayanappa, A., Chalapathi, P. and Maligeppagol, M., 2023. CRISPR/Cas9-mediated mutagenesis of sex lethal (Sxl) gene impacts fertility of the fall armyworm, Spodoptera frugiperda (JE Smith)(Lepidoptera: Noctuidae). Archives of Insect Biochemistry and Physiology, 114(2), pp.1-15. (IF: 2.05, NAAS: 7.50)
  10. Yogi, D., Ashok, K., Anu, C.N., Shashikala, T., Pradeep, C., Bhargava, C.N., Parvathy, M.S., Jithesh, M.N., Manamohan, M., Jha, G.K. and Asokan, R., 2024. CRISPR/Cas12a ribonucleoprotein mediated editing of tryptophan 2, 3-dioxygenase of Spodoptera frugiperda. Transgenic Research, 33(5), pp.369-381. (IF: 2.7, NAAS: 8.70)